Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 11;7:67. doi: 10.1186/s12920-014-0067-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Manase et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Inter-sample variability of observed read depths. Plot of the 95% confidence interval per captured CCDS base across samples for the Agilent 44MB_V2 (green) 50MB_V3 (purple), 51MB_V4 (orange), and 50MB_V5 (blue) capture kits. For V2, V3, and V4 kit versions, over 86% of captured bases varied less than ±5 reads between samples, with the largest variation coming at higher read depths. Given that V5 had only six exomes, the variability is more pronounced due to the small sample size, however, over 85% of captured bases varied by more than ±10 reads between samples. The largest variability was seen at higher read depths (>30X). The depth of sequencing and version of the capture kit did not affect inter-sample variability.