Table 2.
Primers
| Primer name | Primer sequence (5' to 3') | Length | Characteristics | |
|---|---|---|---|---|
| ESA_02131 | F | CATCGGCCGACAGGCCAGTCAATGAATGATGC | 32 | EagI cut site |
| R | CATTCTAGAGAGTACAACGGAATGCGGGG | 29 | XbaI cut site | |
| ESA_01706 | F | CATTCTAGAGTCGGGCGGCTCTTTATCTGG | 30 | XbaI cut site |
| R | CATGGATCCTTGACCAGATGACGCAGTCTTTC | 32 | BamHI cut site | |
| Chimeric-01706 | F | AATAAGCTTGTGGCTTTTTATGCCGGGCTGC | 31 | HindIII cut site |
| R | CAGGCCAGTAATCAGCGCCGCGCCCATGAC | 30 | 3' SOEing overhang | |
| Chimeric-02131CTE | F | CGCGGCGCTGATTACTGGCCTGACGATGAAAG | 32 | 5' SOEing overhang |
| R | AATGGATCCTTACTCGTTAATACGAGGATGCTGG | 34 | BamHI cut site | |
| pUC18 MCS Check | F | CATTAGCTCACTCATTAGGCACC | 23 | pUC18 insert check |
| R | CATTGTAAAACGACGGCCAGTG | 22 | pUC18 insert check |