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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Aquat Toxicol. 2014 Nov 26;0:192–201. doi: 10.1016/j.aquatox.2014.11.016

Figure 6.

Figure 6

AHR2b CRISPR-Cas9 off-target analysis. A. Nucleotide sequence alignment of the AHR2b CRISPR-Cas9 target sequence with the corresponding regions of closely related genes. AHR1a, AHR2a, AHR1b, and AHRR have 4, 4, 6, and 3 mismatches to AHR2b in this region, respectively. B. Surveyor nuclease detection of mutations in AHR1a, AHR2a, AHR1b, and AHRR in the AHR2b CRISPR-Cas9 target region. Digested fragments are indicated by a *. Each lane represents a pool of 5 embryos from which genomic DNA fragments were amplified. U: uninjected control, 1–4: CRISPR-Cas9 injected embryos. AHR1a, AHR2a, and AHRR PCR products from uninjected and injected embryos were sequenced.