Table 2.

AHR2a and AHR2b target regions and oligonucleotides used in ZFN and CRISPR-Cas9-based mutagenesis. The ZFN target site is shown in capital letters. The sequence flanking the target sites are recognized by the ZFN proteins.

Name	Description	Sequence
AHR2a exon 2	ZFN target site	ctgcgcctcagcgtgGGATAcctgagggtcaagag
AHR2a exon 3	ZFN target site	gactcattcagcttcTCTGAaggagagctgctgct
AHR2a exon 2	CRISPR-Cas9 target site	GGAGCGAATCTCATCAGGGA
AHR2a exon 3	CRISPR-Cas9 target site	GGGTGGCTCCTGGAGTCAAC
AHR2b exon2	CRISPR-Cas9 target site	GGACCGTCTGAACGGCGAGC
AHR2b exon3	CRISPR-Cas9 target site	GGGCGAAGGTCTGAGCGGTC
2-ex3Fwd	AHR2a ZFN pair3 screening	TGTTTCACCTCCTGCACAGCTTCC
1XRR1	AHR2a ZFN pair3 screening	TACTAGCTAAACGTGACCTGTCG
2a-ex3CrF	AHR2a CRISPR-Cas exon3 screening	CTGTTCTATCAGGTGGAGAGTCC
2a-ex3CrR	AHR2a CRISPR-Cas exon3 screening	GTTGTTGGATGAGTATGCAGGTGG
AHR2b-CrF	AHR2b CRISPR-Cas9 exon2 screening	AATCTTTGATCCGGTCTGATCTGG
AHR2b-CrR	AHR2b CRISPR-Cas9 exon2 screening	AGACACAACCAAGCAGCAACACG
2bex3-Fwd	AHR2b CRISPR-Cas9 exon3 screening	ATCGCCCAGCCTTAGCTTCCAGTCG
2bex3-Rev	AHR2b CRISPR-Cas9 exon3 screening	GCGTTTTTGCTTTGTCTGCTGTCGG
Fh2A-ex3-CR1	AHR2a exon3 gRNA oligo1	TAGGGTGGCTCCTGGAGTCAAC
Fh2A-ex3-CR2	AHR2a exon3 gRNA oligo2	AAACGTTGACTCCAGGAGCCAC
FhAHR2b-CR1	AHR2b exon2 gRNA oligo1	TAGGACCGTCTGAACGGCGAGC
FhAHR2b-CR2	AHR2b exon2 gRNA oligo2	AAACGCTCGCCGTTCAGACGGT
Fh2B-ex3-CR1	AHR2b exon3 gRNA oligo1	TAGGGCGAAGGTCTGAGCGGTC
Fh2B-ex3-CR2	AHR2b exon3 gRNA oligo2	AAACGACCGCTCAGACCTTCGC
2b-1aF	AHR1a off-target screening	GCTTAATCCTGCTTTCGTCTCTGC
Fh1a-ex2rev	AHR1a off-target screening	AAGAAGTGTTTGGCTCGTAGGTAGC
2b-2aF	AHR2a off-target screening	TTTTACCCCTCACGGAAGAAGATGG
Fh2a-ex2rev	AHR2a off-target screening	GTAGCTCTTGACCCTCAGGTATCC
2b-1bF	AHR1b off-target screening	TTTCCTCCTCTCCAGAGCCAAACC
Fh1b-ex2rev	AHR1b off-target screening	AAGAAGCTCTTGGTGCGCAGGTAGC
RR-ex2fwd	AHRR off-target screening	CTCATCCCTTCTGCCGTCTCGTCC
RR-ex2rev	AHRR off-target screening	AAGAAGCTTTTGACGCGGAGGTAGG