Abstract
The lac repressor protects the lac operator against digestion with deoxyribonuclease. The protected fragment is double-stranded and about 27 base-pairs long. We determined the sequence of RNA transcription copies of this fragment and present a sequence for 24 base pairs. It is: 5′--T G G A A T T G T G A G C G G A T A A C A A T T 3′ 3′--A C C T T A A C A C T C G C C T A T T G T T A A 5′ The sequence has 2-fold symmetry regions; the two longest are separated by one turn of the DNA double helix.
Keywords: regulation, protein-nucleic acid interaction, DNA-RNA sequencing, oligonucleotide priming
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Selected References
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- Beyreuther K., Adler K., Geisler N., Klemm A. The amino-acid sequence of lac repressor. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3576–3580. doi: 10.1073/pnas.70.12.3576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bourgeois S., Riggs A. D. The lac repressor-operator interaction. IV. Assay and purification of operator DNA. Biochem Biophys Res Commun. 1970 Jan 23;38(2):348–354. doi: 10.1016/0006-291x(70)90719-9. [DOI] [PubMed] [Google Scholar]
- Bram S. Secondary structure of DNA depends on base composition. Nat New Biol. 1971 Aug 11;232(2):174–176. doi: 10.1038/newbio232174a0. [DOI] [PubMed] [Google Scholar]
- Downey K. M., So A. G. Studies on the kinetics of ribonucleic acid chain initiation and elongation. Biochemistry. 1970 Jun 9;9(12):2520–2525. doi: 10.1021/bi00814a019. [DOI] [PubMed] [Google Scholar]
- Gilbert W., Müller-Hill B. Isolation of the lac repressor. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891–1898. doi: 10.1073/pnas.56.6.1891. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert W., Müller-Hill B. The lac operator is DNA. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415–2421. doi: 10.1073/pnas.58.6.2415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilbert W. The lac repressor and the lac operator. Ciba Found Symp. 1972;7:245–259. doi: 10.1002/9780470719909.ch14. [DOI] [PubMed] [Google Scholar]
- JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
- Maizels N. M. The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3585–3589. doi: 10.1073/pnas.70.12.3585. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniatis T., Ptashne M. Multiple repressor binding at the operators in bacteriophage lambda. Proc Natl Acad Sci U S A. 1973 May;70(5):1531–1535. doi: 10.1073/pnas.70.5.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pirrotta V. Isolation of the operators of phage lambda. Nat New Biol. 1973 Jul 4;244(131):13–16. doi: 10.1038/newbio244013a0. [DOI] [PubMed] [Google Scholar]
- Ptashne M. ISOLATION OF THE lambda PHAGE REPRESSOR. Proc Natl Acad Sci U S A. 1967 Feb;57(2):306–313. doi: 10.1073/pnas.57.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ptashne M. Specific binding of the lambda phage repressor to lambda DNA. Nature. 1967 Apr 15;214(5085):232–234. doi: 10.1038/214232a0. [DOI] [PubMed] [Google Scholar]
- Riggs A. D., Suzuki H., Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. doi: 10.1016/0022-2836(70)90219-6. [DOI] [PubMed] [Google Scholar]
- Sadler J. R., Smith T. F. Mapping of the lactose operator. J Mol Biol. 1971 Nov 28;62(1):139–169. doi: 10.1016/0022-2836(71)90136-7. [DOI] [PubMed] [Google Scholar]
- de Wachter R., Fiers W. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA. Anal Biochem. 1972 Sep;49(1):184–197. doi: 10.1016/0003-2697(72)90257-6. [DOI] [PubMed] [Google Scholar]