Skip to main content
. Author manuscript; available in PMC: 2014 Dec 21.
Published in final edited form as: Methods Enzymol. 2014;537:47–73. doi: 10.1016/B978-0-12-411619-1.00004-5

Figure 4.

Figure 4

Example Images and Graph of Cell Profiler Adipocyte Size Analysis. (A) Brightfield image of H&E stained paraffin sectioned WAT. (B) Fluorescent image of H&E stained paraffin sectioned WAT using the Texas Red filter cube. (C) Image from (B) outputted from Cell Profiler 2.0 following adipocyte pixel area analysis. Yellow arrows indicate improperly gated cells that must be manually excluded from the dataset. (D) Adipocyte cell size distribution in WAT depots isolated from standard- (SD) and high fat-diet (HFD) fed mice following Cell Profiler 2.0 analysis and conversion of measured adipocyte pixel area to adipocyte diameter as described in section 3.3.

HHS Vulnerability Disclosure