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Immunofluorescent staining of frozen sectioned WAT. (A) A classic crown-like structure stained with antibodies for F4/80 and Ki67 and counterstained with DAPI. (B) Sympathetic innervation of a beige adipocyte as shown through staining with antibodies for UCP-1 and the sympathetic nerve fiber marker tyrosine hydroxylase (TH) and counterstained with DAPI. Scale bar is 20 µm.
