Table 1.
Commonly used fluorescent stains, antibodies, and reporter proteins for whole mount confocal imaging of adipose tissue.
| Antibody / Stain | Fluorochrome / Reporter Protein |
Company, Catalog # | Excitation Laser |
Emission Filter |
|---|---|---|---|---|
| N/A | dTomato | N/A | 543 nm | 590–650 nm |
| N/A | eGFP | N/A | 488 nm | 505–540 nm |
| HCS LipidTox1 | “Green” | Invitrogen, H34475 | 488 nm | 505–540 nm |
| HCS LipidTox1 | “Deep Red” | Invitrogen, H34477 | 633 nm | 645–700 nm |
| Isolectin GS-IB41 | Alexa Fluor 488 | Invitrogen, I21411 | 488 nm | 505–540 nm |
| Isolectin GS-IB41 | Alexa Fluor 647 | Invitrogen, I32450 | 633 nm | 645–700 nm |
| Cell Mask2 | “Orange” | Invitrogen, C10045 | 543 nm | 565–585 nm |
| CD453 | Alexa Fluor 647 | Biolegend, 103123 | 633 nm | 645–700 nm |
| F4/803 | Alexa Fluor 647 | Biolegend, 123121 | 633 nm | 645–700 nm |
| CD11b3 | Brilliant Violet 421 | Biolegend, 101235 | 405 nm | 420–470 nm |
| CD243 | Brilliant Violet 421 | Biolegend, 101825 | 405 nm | 420–470 nm |
| DAPI4 | “Blue” | Invitrogen, D1306 | 405 nm | 420–470 nm |
1
stain at 1:100 in PBS for 1 hour
2
stain at 1:2000 in PBS for 1 hour
3
stain at 1:100 for 24 hours
4
stain at 1:10,000 in PBS for 1 hour