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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Glia. 2013 Dec 6;62(2):259–271. doi: 10.1002/glia.22604

Figure 6. Effect of ARSB silencing on sGAG and C4S-GAG levels in astrocytes.

Figure 6

Primary rat cortical astrocytes were transfected with a non-target (NT siRNA) or an ARSB specific siRNA (ARSB siRNA) using lipofectamine RNAiMAX. Forty-eight hours after transfection, sGAG levels were quantified in astrocyte cell lysates (A); the levels of C4S-GAGs were quantified in astrocyte cell lysates after C4S immunoprecipitation with mouse monoclonal antibodies clone 4D1 (B) from Santa Cruz and Ly111 (C) from Amsbio. **: p< 0.01; ***: p< 0.001 compared with control by the Student’s t-test (n=3).