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. Author manuscript; available in PMC: 2016 Jan 3.
Published in final edited form as: Vaccine. 2014 Nov 24;33(2):298–306. doi: 10.1016/j.vaccine.2014.11.028

Figure 3. TNF-α levels were not affected by depletion of inflammasome-related genes.

Figure 3

THP-1(1×106) knockdown (KD) cells were incubated in 6-well plates with RPMI 1640 media. LPS (100 ng/ml) was added as stimulator. Culture supernatants were collected 24 hrs post-incubation, and TNF-α was measured by ELISA. TNF-α levels from WT cells were compared to KD cells stimulated by LPS. The values for the WT and KD cells were not statistically significant: p < 0.5 for WT vs caspasd-1 KD, and p < 0.5 for WT vs Syk KD cells.