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. Author manuscript; available in PMC: 2016 Jan 3.
Published in final edited form as: Vaccine. 2014 Nov 24;33(2):298–306. doi: 10.1016/j.vaccine.2014.11.028

Figure 5. Uptake of PmpG-1-vaults and colocalization within the endocytic pathway.

Figure 5

(A) THP-1 cells were grown on 18 mm glass cover slips and treated with 30 ug of DyLight 650 labeled PmpG-1-vaults for 15, 30, and 60 min and imaged by confocal microscopy. For immunofluorescence staining, THP-1 cells were reacted with either anti-EEA1 mouse mAb, anti-Lamp1 mouse mAb, or anti-LC3 mouse mAb followed by Alexa Fluor 488-conjugated goat anti-mouse to identify endocytic compartments. (B) Colocalization of PmpG-1-vaults within each compartment was determined by calculation of the Pearson’s correlation coefficient of the red and green channels using ImageJ.