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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunol. 2014 Nov 26;194(1):438–445. doi: 10.4049/jimmunol.1401344

Figure 4. ST2 expression was regulated by T-bet and Eomes in vivo and required for CD8+ T cells and NK cell infiltration of IL-33-expressing tumors.

Figure 4

(A) 2×105 B16-IL-33 cells were injected i.d. into B6 and T-bet−/− Eomes−/− (DKO) mice. 20 days after inoculation, tumors were harvested and processed to generate a single cell suspension. The surface expression of ST2 was analyzed by flow cytometry. Results are mean ±SEM of three independent experiments. ***P<0.001, two-tailed unpaired Student’s t-test. (B) 1×105 4T1-vector or 4T1-IL-33 cells were injected into the mammary fat pad of BALB/c and ST2−/− mice. 20 days after inoculation, tumors were harvested and processed to generate a single cell suspension. Representative flow cytometric plots showing CD8+ T and NK cells in tumor (TIL).