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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunol. 2014 Dec 1;194(1):291–306. doi: 10.4049/jimmunol.1401447

FIGURE 5.

FIGURE 5

SIRT1 protein expression is significantly reduced in the colon of chronic SIV-infected (A-B) compared to uninfected control macaques (D-E). SIRT1 protein localized to both cytoplasm and nuclei of colonic epithelium and LPLs (C). Panels A-B and D-E contain a single label (SIRT1) in green (Alexa 488). Panel C involves double labels with SIRT1 in green (Alexa 488) and Topro-3 for nuclear labeling in far-red (Alexa 647). Colocalization of green (SIRT1) and far-red (Topro-3) appear light yellow. All panels are shown at 40X magnification. Quantitation of cells and regions of interest (ROI) labeled by anti-SIRT1 antibody using Volocity 5.5 software revealed significantly decreased SIRT1 protein expression in the colonic epithelium and LPLs of chronic SIV-infected compared to uninfected control macaques (F). Several ROI were hand drawn on the epithelial and LPL regions in the images from colon. Data were analyzed using non-parametric Wilcoxon’s rank sum test.