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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: J Immunol. 2014 Dec 1;194(1):291–306. doi: 10.4049/jimmunol.1401447

FIGURE 9.

FIGURE 9

In situ localization of miR-34a in the colon of two SIV-infected (A-B) and two uninfected control macaques (D-E) with wild type miR-34a or scrambled probe (C). Panels A, B, D and E involve double labels with miR-34a (red) and IgA (green) for plasma cells. The grey channel represents differential interference contrast (DIC) to reveal tissue architecture. Colocalization of green (IgA) and red (miR-34a) appear light yellow. Note the intense miR-34a staining in the epithelium and markedly increased numbers of IgA/miR-34a++ plasma cells (white arrow heads) in the colon of SIV-infected macaques (A-B). In contrast, miR-34a staining is weak in the colonic epithelium and LPL of the control macaques (D-E). The scrambled probe did not yield a signal (C). All panels are shown at 40X magnification. Quantification of cells and regions of interest (ROI) labeled by LNA-modified miR-34a probe was performed using Volocity 5.5 software after capturing images on a Leica confocal microscope. Several ROI were hand drawn on the epithelial and LPL regions in the images from colon (F). Data was analyzed using non-parametric Wilcoxon’s rank sum test.