Table 1.
Animal IDs, duration and route of SIV infection, viral loads and intestinal histopathology in chronic SIV-infected macaques.
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|---|---|---|---|---|---|---|---|
| Animal ID |
Duration of infection (days post infection) |
Route of Infection |
Viral load- Plasma Copies/mL (106) |
Viral load- Colon Copies/mg RNA (106) |
Histopathology (colon) |
Histopathology (Jejunum) |
Colon Histo- pathology score |
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| Chronic SIV-Infected | |||||||
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| FE53* | 140 | I/rectal | 0.3 | 5 | Mild colitis | ND | 1 |
| FT11* | 145 | I/v | 500 | 2075 | Moderate colitis | Mild enteritis | 2 |
| GH25* | 148 | I/v | NA | 300 | Mild suppurative colitis | Mild enteritis | 1 |
| CP47* | 151 | I/v | NA | 0.9 | Mild colitis | ND | 1 |
| HI68* | 158 | I/vag | 8 | 3 | Mild colitis | ND | 1 |
| HB31* | 180 | I/v | 3000 | 200 | Lymphoid hyperplasia | Lymphoid hyperplasia | 1 |
| HB48* | 180 | I/v | 20 | 0.8 | ND | Mild enteritis | 0 |
| GK31* | 180 | I/v | 30 | 3 | ND | ND | 0 |
| GA19* | 180 | I/v | 100 | 600 | Lymphoid hyperplasia | ND | 1 |
| DV42* | 226 | I/vag | 0.1 | 1 | Mild colitis | ND | 1 |
| HG45* | 281 | I/vag | 0.9 | 0.3 | ND | ND | 0 |
| HG58* | 286 | I/vag | 0.007 | 0.2 | ND | ND | 0 |
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| HD08 | 90 | I/v | NA | 300 | Moderate colitis/cryptitis | Moderate enteritis | 2 |
| DE50 | 129 | I/vag | NA | 0.3 | Mild colitis | ND | 1 |
| HN15 | 177 | I/vag | NA | 0.3 | Granulomatous colitis | Granulomatous enteritis | 3 |
| HL01 | 180 | I/vag | 7 | 0.8 | ND | Mild Amyloidosis | 0 |
| HF27 | 180 | I/v | 40 | 0.04 | Lymphoid hyperplasia | Lymphoid hyperplasia | 1 |
| HV95 | 180 | I/v | 10 | 0.1 | Moderate Colitis | ND | 2 |
| FE24 | 271 | I/v | 3.1 | 0.3 | Mild colitis | ND | 1 |
| EI15 | 300 | I/v | 0.4 | 0.9 | Mild colitis | Mild enteritis | 1 |
| R191 | 786 | I/v | NA | NA | Severe colitis | ND | 3 |
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| Uninfected Controls | |||||||
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| EL66* | NA | NA | NA | NA | NA | NA | 0 |
| EH70* | NA | NA | NA | NA | NA | NA | 0 |
| EH80* | NA | NA | NA | NA | NA | NA | 0 |
| FK25* | NA | NA | NA | NA | NA | NA | 0 |
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| GT20 | NA | NA | NA | NA | NA | NA | 0 |
| IC52 | NA | NA | NA | NA | NA | NA | 0 |
| HT74 | NA | NA | NA | NA | NA | NA | 0 |
| IH95 | NA | NA | NA | NA | NA | NA | 0 |
| IT62 | NA | NA | NA | NA | NA | NA | 0 |
| IT13 | NA | NA | NA | NA | NA | NA | 0 |
| EJ34 | NA | NA | NA | NA | NA | NA | 0 |
| HF54-Pre | NA | NA | NA | NA | NA | NA | 0 |
| HR42-Pre | NA | NA | NA | NA | NA | NA | 0 |
| HD08-Pre | NA | NA | NA | NA | NA | NA | 0 |
| HE68 | NA | NA | NA | NA | NA | NA | 0 |
| HP84 | NA | NA | NA | NA | NA | NA | 0 |
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*
Colon tissue from 12 chronic SIV-infected (FE53-HG58) and 4 uninfected control (EL66-FK25) macaques shown above the dotted line was used for TLDA miRNA profiling. Colon tissues from all SIV-infected (with exception of CP47, HG58 and R191) and 9 uninfected (GT20-HR42) were used for miRNA qRT-PCR confirmation studies Colon tissues from R191 and three uninfected controls (HD08-Pre, HE68 and HP84) were used for immunofluorescence studies. I/v- Intravenous, I/vag- Intravaginal, I/rectal- Intrarectal , NA- Not applicable for Uninfected controls and Not available for Chronic SIV-infected macaques, ND- None detected