Table I.

Summary of histological observations of M-TRAF3^−/− mice at the age of 15–22 months

Mice examined (n=22)	Number	Organs involved
Tumor
Histiocytic sarcoma	2	Spleen, liver
Hepatocellular adenoma	1	Liver
B cell lymphoma (DLBCL or FL)	9	Spleen, CLN, MLN, liver
Inflammation	6	Liver, GI tract, lung, kidney, pancreas, heart
Infection	3	GI tract, liver, lung
Unknown
hemorrhage	7
blister	2
Normal	7

Histologic diagnoses were made based on established criteria (23–28). Mice with DLBCL and FL were diagnosed histologically using criteria outlined in a consensus nomenclature of mouse lymphoid neoplasms (23). Malignancies of histiocytes (histiocytic sarcoma) were diagnosed histologically as described (24). Inflammation was diagnosed histologically using criteria of myeloid cell/lymphocyte infiltration in tissues as described (25). Cases diagnosed with inflammatory conditions exhibited expansions of immature myeloid cells, monocytes and granulocytes in tissues outside the bone marrow and often in spleen or liver. Infections were diagnosed with evidence for bacterial or parasitic infection in different tissues, including visualization of bacteria and entamoeba as described (26–28).

Individual mice could have more than one type of pathology. Examples include B cell lymphoma and lung inflammation, pancreatitis and hepatocellular adenoma, bacterial infection and hemorrhagic liver.