(A) Representative flow plots of no-protease control PBMC stained with control rabbit IgG (black), and for collagen (green), and protease-treated PBMC stained for collagen (red). PBMC were exposed to 12.5 ng/ml (B) tryptase or (C) thrombin for 5 days, resuspended, stained for collagen, and assayed by flow cytometry. Values are mean ± SEM, n=5, * indicates p < .05 (t-test).