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Low pH requirement of AKAV penetration. LB9.K and Vero cells were pretreated with NH4Cl or BafA1. After 1 hr of treatment, cells were infected with AKAV and were incubated in the presence of drugs for 6 hr. Real-time qRT-PCR values to measure intracellular AKAV RNAs are expressed as percentages relative to untreated virus-infected cells. Values represent the mean and SD of three independent experiments. Statistical significance is indicated by *** (P<0.005) (Student’s t-test).
