Fig. 5.

Requirement of clathrin-endocytosis in entry of AKAV strains. LB9.K and Vero cells were pretreated with CPZ for 1 hr, followed by infection with either AKAV OBE-1 (genogroup II) or Iriki (genogroup I) strains in the presence or absence (control) of drug for 6 hr. Real-time qRT-PCR values to measure intracellular AKAV RNAs are expressed as percentages relative to untreated virus-infected cells. Values represent the mean and SD of three independent experiments. Statistical significance is indicated by *** (P<0.005) (Student’s t-test).