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Analysis of recombinant SLURP-2. (A–B). The SLURP-2 refolding efficiency depends on the pH of the refolding buffer (A) and the concentration of the reduced (GSH) and oxidized (GSSG) forms of glutathione (B). The peak corresponding to the refolded SLURP-2 is denoted by an asterisk. (C). SDS-PAGE analysis of the refolded SLURP-2 after purification by HPLC. (D). Mass-spectrum of the refolded SLURP-2
