Fig. 1.

Multistep gating of monocyte subsets. (A) Identification of the monocyte region based on the forward scatter (FSC) and side scatter (SSC) parameters; (B) Gating of the total population based on CD14 positive events; (C) Identification of monocyte subsets by the expression level of the surface markers CD14 and CD16; (D–F) Gating in the analysis of the expression of various receptors and membrane rafts on monocyte subsets based on the SSC parameters and positive events (illustrated by an example of the GM1 raft marker)