Skip to main content
. Author manuscript; available in PMC: 2014 Dec 22.
Published in final edited form as: Methods Mol Biol. 2012;917:69–110. doi: 10.1007/978-1-61779-992-1_5

Figure 2. Haploid and gynogenetic embryos.

Figure 2

Wild type eggs were fertilized using homozygous cardiac actin-RFP transgenic sperm, either untreated to form conventional diploid embryos (A) or UV-irradiated to form haploids (C). Paternal transgene transmission is visible in the diploid clutch (B) but absent in the haploids (D). Haploids can form anterior structures well, but posterior structures are truncated. Note that three spontaneously diploidized embryos without paternal transgene appear in the haploid clutch. Panels E-G show the karyotype of outcrossed diploid (E), haploid (F) and early cold shock gynogenetic diploid embryos (G).