FIG. 1.

Morphometric quantification of immunohistochemistry. (A–C) Quantification of glial fibrillary acidic protein (GFAP) expression in the granule cell layer and hilus of the dentate gyrus. Color images of GFAP immunolabeling (A) were converted to black and white (B) and then inverted in Adobe Photoshop. The dentate gyrus (DG) was subdivided into the granule cell layer (GCL) and the hilar regions, and immunopositive area was quantified (C) using MCID software. (D–F) Quantification of BrdU and GFAP expression in the subventricular zone (SVZ) proper, white matter dorsal to SVZ, and the striatum below the SVZ. The regions of interest were outlined using MCID software on DAPI-stained sections images taken at 10× and using the parameters indicated in (D). A line separating the striatum from white matter was drawn in linked images from GFAP-stained sections (E) and GFAP immunopositive proportional target area quantified in the three regions of interest. Then BrdU proportional target area was quantified in linked images from BrdU-stained sections (F). (G–I) Quantification of density of Iba-1-labeled cells in the SVZ. The SVZ was outlined using Axiovision software on DAPI-stained sections images taken at 10× and (G) linked to Iba-1-stained images (H, I) in which the number of Iba-1-labeled cells (I, arrow) in the SVZ were counted.