FIG. 10.
Apotome images (60×) of fixed coronal brain sections from ipsilateral stroke-injured hemispheres of ligated mice treated with 1×105 CBSC-dosage of CD34+-enriched fraction of human CB cells or vehicle (Plasma Lyte A) stained with human nuclear antibody (A and E, green), human CD34+ (B and F, red), and Dapi (arrow C and G, blue); merged images (D and H) demonstrate that double-labeling is imaging the same cell. Nonspecific labeling with antibodies was noted in the injured hemispheres of both CBSC (A–D) and vehicle-treated (E–H) animals.