FIG. 1.

Establishment of CBMSC cell cultures. In T0 culture, it is frequent to observe adherent cells showing different morphologies that we do not recognize as MSCs (A). Among them, (B) nonproliferating fibroblast-like cells (indicated by arrows) and polynucleated (two to seven distinguishable nuclei) cells with a very large cytoplasm (dashed circles). (C) The pie chart at the left represents total isolation experiments relying on immunodepletion of nonmesenchymal cells; the right pie chart summarizes culture outcomes of CB units that gave rise to primary colonies of stromal cells (positive events). CBMSCs, cord blood-derived multipotent mesenchymal stromal cells; MSCs, mesenchymal stromal cells.