FIG. 2.

CBMSC morphology and growth kinetics. Adherent and proliferative cells isolated from processed CB units possess distinct morphology and cell shape. The images were taken from a representative CBMSC population and show subconfluent cells at early passages (P1, P4; A) and in long-term culture (P8, P12; B) at the indicated magnitudes. Representative growth trends of CBMSCs grouped by similar CPD values (vertical axis) and longevity (horizontal axis) are presented in (C, D). In the boxes, the means (black rectangle) and deviation standards (vertical bars) of CPDs for each passage are also shown. The values obtained fitted almost perfectly with a linear function in the case of LL-CBMSCs (y=3.0x−0.3), whereas in the case of SL-CBMSCs, the growth was slower at the beginning and the arrest was gradual starting from the last passages, so that the mean CPD values better fitted a polynomial curve (y=−0.1x³+1.1x²−0.9x+1.6). CPD, cumulative population doublings cut-off; LL-CBMSCs, long-living CBMSCs; SL-CBMSCs, short-living CBMSCs.