Figure 3.

Adherence of 100 nm NPs to Caco-2 cells. The glycocalyx was counter-stained with a lectin (red) to visualize the cell surface of the monolayer. Stacks of 2-dimensional microscope images were processed with the software Imaris x64 to give a 3D rendition. A: In the absence of BSA, NPs are attached to the cell surface mainly in form of particle agglomerates (green spots in variable size). B: After preincubation of NPs with BSA only few and small particle spots are visible. Scale bar: 15 µm.