Figure 1.

Non-viral vectoring of DEAE–dextran and DEAE–dextran–MMA graft copolymer (DDMC). (a) Transfection of DDMC into HEK293 cell lines. The grafting rate is 130% for sample 2 at 10 mg/mL, sample 3 at 20 mg/mL, and sample 4 at 28.6 mg/mL. Expression of the LacZ gene is shown at 48 h after transfection. (b) DNase degradation (1: DEAE–dextran, 2 DDMC): To the samples was added 4 mL of distilled water, then 10 u of DNase (RQ1 RNase-free DNase, Promega) and 0.1 mL of 10× reaction buffer (400 mM Tris-HCl, 100 mM MgSO₄, 10 mM CaCl₂, pH 8) and the samples were incubated at 37 °C. The wavelength used for this experiment was 633 nm for toluidine blue isolated from DNA. (b): Reprinted from [18].