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. 2014 Dec 1;111(50):17827–17832. doi: 10.1073/pnas.1420096111

Fig. 1.

Fig. 1.

SWI/SNF interacts with Sir3p. (A) SWI/SNF, but not RSC, interacts with resin-bound Sir3p. Purified remodeling enzyme was incubated with anti-FLAG resin that was prebound with (+) or without (−) Sir3p. B, bound fraction; U, unbound supernatant. (B) Subunits of SWI/SNF, but not RSC, interact with Sir3p by far Western. Equimolar amounts of SWI/SNF, RSC, and Sir2p/4p complex were separated on SDS/PAGE, electroblotted, renatured, and incubated with Sir3–FLAG. Sir3p-bound protein bands were visualized by anti-FLAG immunoblotting.