Fig. 3.

SWI/SNF ATPases interact with BAH core domains. (A) Schematic shows C-terminal truncations within the Sir3p BAH domain. The indicated GST–BAH fusion proteins were incubated with either SWI/SNF or RSC, and bound fractions were assayed by Western. The Rsc2p BAH fusion contains only the core BAH domain; the BAH–CT-1 fusion also contains the C-terminal conserved CT-1 domain from Rsc2p. Western analyses used sera to the Arp9p subunit, common to both remodeling enzymes. (B) SWI/SNF, RSC, or Isw2 complexes were incubated with GST–BAH fusions from yeast Orc1p or Sir3p. Bound fractions were assayed by Western to the indicated subunits. Lower shows Ponceau-stained membrane, depicting levels of GST fusions.