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. 2014 Dec 1;111(50):18061–18066. doi: 10.1073/pnas.1411294111

Fig. 4.

Fig. 4.

Ind-Cl increases survival, proliferation, migration, and differentiation of OPC in the SVZ and CC of EAE mice. Improved PLP_EGFP+ cell numbers in animals pre- and posttreated with Ind-Cl were observed (A and D). White dashed boxes within the normal CC (A) depict areas examined at 40× magnification in B and C. Ind-Cl treatment improved numbers of olig2 (red)-stained cells (B and F). Increase in adenomatous polyposis coli (CC1; red) immunostaining revealed improved mature OL numbers in all Ind-Cl-treated groups (C and E). Costaining with olig2 (red) and platelet-derived growth factor α (inset; red) was used to determine the percentage of OPCs (G and H). Representative 20× magnification images of coronal brain slices containing CC adjacent to the SVZ are shown, with the dashed box indicating the region imaged at 40× magnification and represented in the inset (G). Prophylactic or therapeutic Ind-Cl treatment increased the percentage of proliferating Ki67+ and olig2+ OPCs near-SVZ CC compared with the vehicle-treated group. n = 8 mice/group; *P < 0.05, **P < 0.01, ANOVA.