Fig. 4.

Changes in Notch signaling in the artery alone did not affect AV shunt formation. (A–F) Lectin-perfused vasculature on P18 surface cerebral cortex. (A and B) ROSA:LNL:tTA;Notch4* mice in which Notch4* was expressed at P7 with temporally inducible Cre-recombinase. (D–F) Notch4* mutant or control mice with Cre-mediated RBPJ deletion at P7. (A) n = 83 AV connections in three mice. (B) n = 43 AV connections in three mice. (C) n = 103 AV connections in three mice. (D) n = 191 AV connections in five mice. (E) n = 186 AV connections in six mice. (F) n = 171 AV connections in seven mice. Blue arrowheads indicate AV connections. (Scale bars, 100 μm.)