Fig. 1.

Schematic illustration of the experimental setup to measure structural kinetics and solvation dynamics of metalloenzymes. The catalytic domain of human MT1-MMP is shown in the gray cartoon (residues 114–291), and the active site zinc ion is shown in orange. The collagen-like substrate is shown in dark gray. The enzyme and substrate are mixed in a stopped-flow apparatus, and changes in different spectroscopic properties provide information regarding structural dynamic transitions of the enzyme (by fluorescence or XAS), substrate (by CD), or solvent (by KITA).