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Toolkit TF proteins, identified as responsive to social challenge in all three species, localized in mouse brain with immunohistochemistry. Sagittal sections from resident animals were treated with antibodies to EMX1, FOXG1, and NR5A1. Antibodies, stained in red, detected very similar clustered populations of neurons in the ventromedial hypothalamus (VMH); NR5A1 was additionally detected in scattered neurons within the arcuate hypothalamic nucleus (AHN).
