Fig. 4.

Endogenous analysis of CLA in Arabidopsis. Endogenous CLA was detected in WT, the max2 and atd14 mutants by LC-MS/MS [a quadrupole/time-of-flight instrument (QTOF)]. (A) Ion traces from the LC-MS/MS analysis (Left) and product ion spectra (Right) derived from respective precursor ions of [1-¹³CH₃]-labeled (internal standard) and endogenous CLA extracted from max2 roots as a representative data and those of unlabeled authentic standard are shown. (B) Endogenous CLA was quantified in root extracts of WT, max1, max4, atd14, and max2 using [1-¹³CH₃]CLA as an internal standard by LC-MS/MS (QTOF). Data are the means ± SD (n = 3). n.d., not detectable.