Fig. 4.

The developmental arrest in Miz-1–deficient pre-B cells is associated with several p53 effectors. (A) Analysis of p53 target gene expression by RT-qPCR in sorted pro-B cells from WT, Miz-1^ΔPOZ, Trp53^−/−, and Miz-1^ΔPOZ × Trp53^−/− mice. Data represent average fold change over Gapdh from two independent experiments and are presented as mean ± SD. (B) FACS analysis of B cell subsets from BM of WT, Miz-1^ΔPOZ, p53^K117R, and Miz-1^ΔPOZ × p53^K117R mice. FACS plots are a representative example of three independent experiments. (C) Absolute cell counts of B cell subsets from B. Data are averaged from three independent experiments and are presented as mean ± SD.