Fig. 2.

Alternative splicing produces two proteins from the AtPAA1 locus. (A) Schematic of the AtPAA1 and AtPCH1 transcripts. Asterisks mark the location of the paa1-1, paa1-3, and paa1-5 mutations. Exons are colored as in Fig. 1. (B) Immunoblot with antibody raised against the N-terminal HMBD of AtPAA1. Equal loading was confirmed with Ponceau staining of the membrane after transfer. Serial dilutions of Col-0 protein extract (%Col) were performed as indicated.