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Interaction of ΔN-AtPAA1 and ΔN-AtPAA2 with soluble Cu+ chaperones. Cu+ (orange circles) and protein (gray squares) contents of the wash and elution fractions are shown. (A) Cu+ transfer assays between Cu+-AtPCH1 and ΔN-AtPAA1 or ΔN-AtPAA2. (B) Cu+ transfer assays between Cu+-AtPAA1-HMBD and ΔN-AtPAA1 or ΔN-AtPAA2. (C) Cu+ transfer assays between Cu+-AtPAA2-HMBD and ΔN-AtPAA1 or ΔN-AtPAA2. (D) Cu+ transfer assays between Cu+-AtCCS and ΔN-AtPAA1 or ΔN-AtPAA2.
