Fig. 4.

Determination of IgH VDJ recombination across cells and clones clustered by Jaccard distance. (A) Patient 4 represents a pattern seen for three of six patients, with the use of a single VH segment in the rearranged VDJ sequences. (B) Patient 5 had two rearranged alleles detected in both clones. (C) Patient 1 had a significant fraction of VH-replacement clones. In addition, in clone 1 EYA4 mutation closely segregates with VH-segment IGHV3-33*01 (dashed box), suggesting it is a separate clone with a unique IgH sequence. Clone 2 had a much higher rate of VH-replacement, as well as rate of no VDJ calls. (D) Patient 2 clone 4 almost exclusively used IGHV3-64*01, whereas the other clones had high levels of VH replacement and no VDJ sequence calls. Black box represents the VH segment call for the VDJ sequence detected in each cell, whereas the white box represents no call.