Fig. 2.

Relationship of germ line status to pheromone output. Shown are the results of volatile chemotaxis assays of C. elegans males to hermaphrodites of various genotypes, accompanied by a diagram of germ line status in each of the strains used in the mutant survey. Significance of the dataset was determined by ANOVA: F(13,50) = 9.319; P < 0.0001, with significant differences from WT determined by Dunnett’s posttest. *P < 0.05; **P < 0.01. In general, fertilization and volatile pheromone production are anticorrelated. WCM for all mutant strains except aged N2 hermaphrodites was prepared from young adults. Chemotaxis assays for spe-42 and egg-4;egg-5 double mutant were performed six times instead of three times, owing to high variance.