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. 2014 Dec 2;7:544. doi: 10.1186/s13071-014-0544-0

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© Dieme et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MALDI-TOF MS spectra of whole mosquitoes at different aquatic stages ranging from 2 to 20 kDa. A representative spectra from biological replicates performed in quadruplicate of each aquatic developmental stage of An. gambiae (A) and Ae.albopictus (B) specimens is shown. The mosquito life stages are indicated in the right corner of each protein profile spectrum. a.u., arbitrary units; m/z, mass-to-charge ratio; L2 to L4, larval stages 1 to 4; P1, pupae at day 1. (C) Dendrogram of MALDI-TOF MS spectra of different life stages of An. gambiae and Ae. albopictus. Each life stage is represented by 2 distinct specimens. Distance unit correspond to the relative similarity calculated from the distance matrix.