(A) Posture analysis of undisturbed flp-18(gk3063) mutants revealed wild-type-like overall quiescence but reduced correlations between subsequent bouts. R = 0.33 ± 0.06, N = 12 animals. These correlations were significantly different (p < 0.05) from those of wild-type and npr-1 mutants shown in Figures 2A and 5A, respectively. (B) Frame subtraction analysis of flp-18 mutants during L4leth in the presence of weak blue light stimuli (15 s, 20 mW/cm2). All stimuli were initiated at t = 0. The dynamics of locomotion revealed defects in the ability of flp-18 mutants to compensate for the motion induced by the stimulus with enhanced quiescence. Left: the locomotion responses during lethargus of each of the two alleles tested and its wild-type control group shown on a semi-log scale. Shaded area denotes mean ± s.e.m. Asterisks denote that during the trough in locomotion, the fraction of quiescence of the mutant allele was significantly lower than that of its respective wild-type control (p < 0.01). Right: for each strain, the quiescence fraction was calculated during 1 min intervals centered at the times of the peak and trough of the L4leth responses, as well as for their respective pre-stimulus baselines. Plots and bars depict mean ± s.e.m obtained from datasets of N = 40–50 animals per condition. Asterisks and double asterisks denote p < 0.05 and p < 0.01, respectively.
DOI:
http://dx.doi.org/10.7554/eLife.04380.015