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. 2014 Dec 7;12:77. doi: 10.1186/s12964-014-0077-3

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© Du et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression and functional analysis of human IRAK-M-DD mutants in 293T cells. (A) Analysis of expression levels of IRAK-M variants by transfection in 293T cells by Western blotting performed on cell lysates with an antibody directed to the C-terminal of IRAK-M. (B) Effect of IRAK-M-DD mutations on NF-κB activation by overexpression in 293T cells. (C) Effect of IRAK-M-DD mutations on A20 mRNA induction by overexpression in 293T cells. (D) Effect of IRAK-M-DD mutations on IL-8 protein and mRNA production by overexpression in 293T cells. (B-D) N=4, mean±SEM. *Indicates difference with WT IRAK-M P<0.05. Shaded bars depict results of IRAK-M molecules with combinations of mutated residues/stretches within the same patch.