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. 2014 Dec 7;12:77. doi: 10.1186/s12964-014-0077-3

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© Du et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of human IRAK-M and death domain mutants in lung epithelial cells. WT IRAK-M and DD-mutants were stably expressed in the lung epithelial cell line H292 by lentiviral transduction and FACS-sorting of EGFP positive cells which is bicistronically expressed. (A) IRAK-M expression evaluated by western blotting. (B) The effect of stable IRAK-M expression and IRAK-M mutants was determined on IL-1β and Flagellin (TLR5) mediated IL-8 expression in the supernatant after 6 hour stimulation. Shaded bars depict results of IRAK-M molecules with combinations of mutated residues/stretches within the same area. N=8, mean±SEM. * indicates difference with WT IRAK-M P<0.05. (C) Effect of stable expression of WT and DD domain mutants on IL-8 mRNA levels induced by Flagellin after 3 hours. N=3, mean±SEM.