Table 2.
Differentially expressed proteins in stacked transgenic maize variety versus controls (single event transgenic maize variety with the same genetic background) and non-genetically modified counterpart and a landrace by 2D-DIGE analysis
| Match ID | Genebank ID | Protein name | Mascot score | Sequence coverage (%) | Peptides | Theor. mass (kDa) | Theor. pI (pH) | Exp. mass (kDa) | Exp. pI (pH) | Fold change (ANOVA P < 0.05) | Biological process (KEGG Orthology) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 55 | gi|11467199 | ATP synthase CF1 beta subunit [Zea mays] | 2248 | 72 | 62 | 54 | 5.31 | 56 | 5.80 | Conv, RR, RRxBt > Bt > Land | Metabolism (energy metabolism) |
| 155 | gi|413948212 | hypothetical protein ZEAMMB73_661450 [Zea mays] | 723 | 44 | 21 | 46 | 5.62 | 44 | 5.96 | Land > Conv, RR, Bt, RRxBt | Metabolism (energy metabolism) |
| 156 | gi|413939324 | glutamate-oxaloacetate transaminase2 [Zea mays] | 1201 | 61 | 43 | 50 | 8.43 | 44 | 6.12 | Land > Bt > Conv, RR, RRxBt | Metabolism (carbohydrate metabolism; biosynthesis of amino acids) |
| 231 | gi|195622374 | fructose-bisphosphate aldolase [Zea mays] | 798 | 40 | 19 | 40 | 5.39 | 37 | 5.50 | Land > Conv, RR, Bt, RRxBt | Metabolism (carbohydrate metabolism; biosynthesis of amino acids) |
| 406 | gi|414591286 | APx2-cytosolic ascorbate peroxidase [Zea mays] | 1036 | 59 | 20 | 31 | 5.77 | 27 | 5.78 | Conv, RR, Bt, RRxBt > Land | Metabolism (carbohydrate metabolism; biosynthesis of amino acids) |
| 426 | gi|226504576 | APx1 - cytosolic ascorbate peroxidase [Zea mays] | 772 | 54 | 18 | 27 | 5.65 | 26 | 5.74 | Bt > Conv > RRxBt > RR > Land | Metabolism (carbohydrate metabolism; biosynthesis of amino acids) |
| 171 | gi|414586172 | 3-isopropylmalate dehydrogenase [Zea mays] | 1042 | 44 | 23 | 43 | 5.62 | 42 | 5.18 | Conv > Bt > Land> RR > RRxBt | Metabolism (biosynthesis of amino acids) |
| 175 | gi|195645514 | acyl-desaturase [Zea mays] | 441 | 24 | 8 | 45 | 6.61 | 42 | 6.15 | Bt > Conv, RR, RRxBt > Land | Metabolism (fatty acid metabolism) |
| 177 | gi|308081433 | coproporphyrinogen III oxidase [Zea mays] | 321 | 24 | 9 | 47 | 7.23 | 42 | 6.12 | Conv, Bt > RR, RRxBt > Land | Metabolism (cofactors and vitamins metabolism) |
| 762 | gi|226499080 | dihydroflavonol-4-reductase [Zea mays] | 534 | 36 | 14 | 35 | 5.43 | 33 | 5.83 | RR > Conv, Bt, RRxBt > Land | Metabolism (biosynthesis of other secondary metabolites) |
| 64 | gi|226492645 | vacuolar ATP synthase subunit B [Zea mays] | 711 | 45 | 17 | 54 | 5.07 | 54 | 5.19 | Bt, RRxBt > Conv, Land, RR | Metabolism (energy metabolism); Cellular Processes (transport and catabolism; phagosome) |
| 105 | gi|162458207 | enolase 1 [Zea mays] | 1604 | 67 | 29 | 48 | 5.20 | 49 | 5.60 | RR > Bt > Conv > RRxBt > Land | Metabolism (carbohydrate metabolism; biosynthesis of amino acids); Genetic Information Processing (Folding, sorting and degradation); Environmental Information Processing (signal transduction) |
| 437 | gi|413951084 | hypothetical protein ZEAMMB73_536198 [Zea mays] | 416 | 32 | 9 | 28 | 5.14 | 26 | 5.39 | Land > Conv > Bt > RR > RRxBt | Metabolism (metabolism of cofactors and vitamins); Genetic Information Processing (transfer RNA biogenesis) |
| 714 | gi|195619804 | enolase [Zea mays] | 663 | 40 | 16 | 48 | 5.59 | 56 | 6.05 | Land > Conv, Bt, RRxBt > RR | Metabolism (carbohydrate metabolism; biosynthesis of amino acids); Genetic Information Processing (Folding, sorting and degradation); Environmental Information Processing (signal transduction) |
| 137 | gi|226505740 | DIMBOA UDP-glucosyltransferase BX9 [Zea mays] | 1197 | 49 | 23 | 50 | 5.15 | 45 | 5.43 | RR > Conv, RRxBt > Bt > Land | Metabolism (biosynthesis of other secondary metabolites); Genetic Information Processing (folding, sorting and degradation) |
| 415 | gi|414591366 | 6-phosphogluconolactonase isoform 1 [Zea mays] | 333 | 28 | 7 | 35 | 7.71 | 26 | 5.08 | Conv, RR, Bt, RRxBt > Land | Metabolism (carbohydrate metabolism; biosynthesis of amino acids) |
| 421 | gi|195611274 | 14-3-3-like protein [Zea mays] | 858 | 67 | 23 | 29 | 4.82 | 26 | 4.93 | Bt, RRxBt > Conv, Land, RR | Environmental Information Processing (signal transduction); Cellular Processes (cell growth and death); Exosome (exosomal protein) |
| 572 | gi|226504688 | uncharacterized protein LOC100272933 precursor [Zea mays] | 202 | 13 | 9 | 22 | 6.02 | 19 | 6.62 | Bt, RRxBt only | Metabolism (carbohydrate metabolism) |
| 345 | gi|195619262 | gibberellin receptor GID1L2 [Zea mays] | 244 | 20 | 5 | 33 | 4.93 | 31 | 5.06 | Bt, RRxBt only | Environmental Information Processing (signal transduction) |
| 545 | gi|195626524 | 2-cys peroxiredoxin BAS1 [Zea mays] | 160 | 23 | 4 | 28 | 5.81 | 21 | 4.56 | Bt, RRxBt only | Cellular Processes (transport and catabolism) |
| 38 | gi|226493235 | LOC100281701 [Zea mays] | 1110 | 41 | 17 | 61 | 5.20 | 59 | 5.15 | RR only | Genetic Information Processing (folding, sorting and degradation) |
| 750 | gi|226530174 | ankyrin repeat domain-containing protein 2 [Zea mays] | 619 | 57 | 16 | 38 | 4.57 | 36 | 4.66 | RR only | Genetic Information Processing (folding, sorting and degradation) |
Proteins were considered differentially modulated at statistical significant difference in normalized volume in stacked vs. single GM events and control samples at ANOVA P < 0.05. Proteins were classified in functional categories based on the ExPASy, KEGG Orthology databases and on careful literature evaluation. The Table reports spot number (Match ID), accession number and protein name, together with Mascot score, sequence coverage, number of matched peptides, theoretical and experimental molecular weight (MW), isoelectric point (pI) and fold change. Abbreviations for each plant variety are provided within ‘Methods’ section.