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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: J Mater Chem B. 2014 Dec 14;2(46):8186–8193. doi: 10.1039/C4TB00957F

Fig 3. Screening of general parameters for stem cell transfection using 3D mineral coated PLG scaffolds.

Fig 3

Mineral coatings were formed in 2×mSBF with 4.2 mM CO32−. (A) Comparison of luciferase activity between PLG and mineral coated PLG scaffold with different transfection approaches. (B) Luminescence intensity from cell culture medium after 2 day culture of C3H10T1/2 cells under various transfection conditions. (C) The influence of pLuc complex amount on Luciferase activity, * indicates significant difference compared to loading amount at 5 µg/well (D) The influence of pLuc complex amount on cell viability, * indicates significant difference compared to loading amount at 5 µg/well (E) The influence of N/P ratio on luciferase activity, * indicates significant difference compared to all other N/P ratios (F) The influence of N/P ratio on cell viability. (G) The influence of cell seeding density on luciferase activity. (H) The influence of cell seeding density on cell viability, * indicates significant difference compared to seeding density at 25K/well