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. Author manuscript; available in PMC: 2015 Dec 14.
Published in final edited form as: J Mater Chem B. 2014 Dec 14;2(46):8186–8193. doi: 10.1039/C4TB00957F

Fig 4. Screening of optimal BMP-2 expression by hMSCs using 3D mineral coated scaffolds.

Fig 4

(A) pBMP-2 complexes binding on mineral coatings. The binding of the complexes was measured after 2 h incubation on the mineral coated scaffolds. (B) Total amount of BMP-2 produced by hMSCs after 72 h transfection of hMSCs. Secreted BMP-2 was found both in medium and on mineral coating (C) BMP-2 detected in the cell culture medium after 72 h transfection of hMSCs by ELISA; only a small portion of secreted BMP-2 was found in medium. (D) BMP-2 detected by dissolving the mineral coating with 200 µL 100 mM EDTA in PBS after 72 h transfection of hMSC by ELISA. The majority of secreted BMP-2 was found in the dissolved mineral coating. # indicates significant differences compared to Lipo2000 * indicates significant differences compared to Lipo2000.