Figure 4. Dual sgRNAs can direct the deletion of large chromosome segments.

(A, D) Schematic of gene structures. (B) dpy-7(-) mutant exhibited a dumpy phenotype. (E) lin-15b/15a genes belong to syn-Muv gene families. Disruption of both lin-15b and lin-15a together results in a Muv phenotype. (C, F) Summary of the microinjection experiments. The dominant transformation marker mCherry was co-injected with Cas9 and sgRNA #1 and #2 or sgRNA #2 and #3 expression plasmids. F1 animals with mCherry expression were transferred to OP50 plates, and F2 animals were scored for dumpy (B) or Muv phenotypes (E). Mutant animals were further PCR amplified and sequenced.