Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | Sources or references |
|---|---|---|
| E. COLI STRAINS | ||
| DH5α | Host strain for plasmids | Lab stock |
| WM3064 | Donor strain for conjugation; ΔdapA | W. Metcalf, UIUC |
| XL1-Blue MRF'Kan | Recipient strain for one-hybrid system | Stratagene |
| S. ONEIDENSIS STRAINS | ||
| MR-1 | Wild-type | ATCC 700550 |
| HG0197 | ΔdesA derived from MR-1 As MR-1 plus ΔkatG-1 | This study |
| HG0198 | ΔfabR derived from MR-1 | This study |
| HG1856 | ΔfabA derived from MR-1 | This study |
| HG2885 | ΔfadR derived from MR-1 | This study |
| HG0197-1856 | ΔfabAΔdesA derived from MR-1 | This study |
| PLASMIDS | ||
| pHGM01 | Suicide vector for mutant construction | Jin et al., 2013 |
| pHG101 | Promoterless vector for complementation | Wu et al., 2011 |
| pHG102 | pHG101 containing PArcA | Wu et al., 2011 |
| pHGEI01 | Integrative lacZ reporter vector | Fu et al., 2014a |
| pBBR-Cre | Helper plasmid for antibiotic marker removal | Fu et al., 2013 |
| pBXcmT | B1H bait vector | Guo et al., 2009 |
| pTRG | B1H target vector | Stratagene |
| pTP247 | His6-tag expression Vector, ApR | Gao et al., 2008a |