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. Author manuscript; available in PMC: 2014 Dec 22.
Published in final edited form as: Nat Med. 2014 Jan 19;20(2):175–183. doi: 10.1038/nm.3450

Figure 6.

Figure 6

Mfge8−/− mice are protected from DIO. (a) Weight gain in Mfge8−/− and Mfge8+/+ mice on a normal chow diet (NCD) (n = 5) or HFD (n = 10). Statistical analysis compares Mfge8−/− and Mfge8+/+ mice on a HFD. (b) eWAT weights in 20-week-old Mfge8−/− and Mfge8+/+ mice on a HFD. n = 5. (c) eWAT Mfge8 expression in 20-week-old Mfge8+/+ mice on a NCD or HFD. (d) Adipocyte size in 20-week-old Mfge8+/+ (left) and Mfge8−/− (right) mice on HFD. Scale bars, 200 µm. (e) Adipocyte number per high-powered field (HPF) in 20-week-old mice on a NCD (n = 5) or HFD (n = 10). (f) H&E stain of liver sections from 20-week-old Mfge8+/+ (left) and Mfge8−/− (right) mice on a HFD. Scale bars, 100 µm. (g) Liver TG concentrations in 20-week-old mice on a NCD (n = 5) or HFD (n = 10). (h) Body composition of Mfge8+/+ and Mfge8−/− mice aged 10 weeks on a NCD (left, n = 23 and n = 24, respectively), aged 20 weeks on a NCD (middle, n = 5) or aged 20 weeks on a HFD (right, n = 10) for 12 weeks. (i) Insulin tolerance tests in 20-week-old Mfge8+/+ and Mfge8−/− mice on a HFD. n = 10. (j) Fecal TG (left, n = 6) and energy content (right, n = 3) in Mfge8+/+ and Mfge8−/− mice on a HFD. Each sample represents stool combined from 2 mice. Male mice were used for all experiments. For all in vivo experiments, each group of 5 mice represents 1 independent experiment. *P < 0.05, **P < 0.01, ***P < 0.001. Data are expressed as mean ± s.e.m. A Student’s t-test was used for statistical analyses.